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Tuesday, June 28 • 4:48pm - 4:55pm
SeqResults - Simple comparisons of results across libraries

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Slides
Video

Authors

  • Brad Langhorst, New England Biolabs
Abstract
We have developed SeqResults to enable simple comparision of libraries across experiments. The Galaxy-integrated component captures metadata and results in a relational database. Results are available via a simple web site and Tableau visualizations. SeqResults has recently been extended with RNA-seq features. It aggregates simple metrics like fractions of reads on exons, introns and other genomic regions, average 5'-3' coverage and alignment efficiency. However, summary metrics are only part of the story.  Accurate representation of transcript levels is important to any RNA-seq experiment. We present a simple interface to compare transcript levels as well as 5'-3' coverage profiles of individual transcripts across experiments. SeqResults now contains millions of individual results from 6841 libraries produced during development of NEBNext library preparation reagents.

Presenters
avatar for Bradley W. Langhorst

Bradley W. Langhorst

New England Biolabs