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Wednesday, June 29 • 3:15pm - 4:30pm
P22: SeqResults for development of RNA-seq reagents

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Poster    doi: 10.7490/f1000research.1112747.1

Authors

Timur Shtatland, NEB; Erbay Yigit, NEB; Keerthana Krishnan, NEB; Mehmet Karaca, NEB; Deyra N. Rodriguez, NEB; Eileen T. Dimalanta, NEB; Theodore B Davis, NEB; Bradley W. Langhorst, NEB

Abstract 
We have developed SeqResults to enable simple comparison of libraries across experiments. The Galaxy-integrated component captures metadata and results in a relational database. Results are available via a simple web site and Tableau visualizations. SeqResults has recently been extended with RNA-seq features. It aggregates simple metrics like fractions of reads on exons, introns and other genomic regions, average 5'-3' coverage and alignment efficiency. However, summary metrics are only part of the story. Accurate representation of transcript levels is important to any RNA-seq experiment. We present a simple interface to compare transcript levels as well as 5'-3' coverage profiles of individual transcripts across experiments. SeqResults now contains millions of individual results from 6841 libraries produced during development of NEBNext library preparation reagents.

Presenters
avatar for Brad Langhorst

Brad Langhorst

Developmenet Group Leader, NEB


Wednesday June 29, 2016 3:15pm - 4:30pm EDT
IMU Solarium

Attendees (3)