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Wednesday, June 29 • 3:15pm - 4:30pm
P22: SeqResults for development of RNA-seq reagents

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Poster    doi: 10.7490/f1000research.1112747.1

Authors

Timur Shtatland, NEB; Erbay Yigit, NEB; Keerthana Krishnan, NEB; Mehmet Karaca, NEB; Deyra N. Rodriguez, NEB; Eileen T. Dimalanta, NEB; Theodore B Davis, NEB; Bradley W. Langhorst, NEB

Abstract 
We have developed SeqResults to enable simple comparison of libraries across experiments. The Galaxy-integrated component captures metadata and results in a relational database. Results are available via a simple web site and Tableau visualizations. SeqResults has recently been extended with RNA-seq features. It aggregates simple metrics like fractions of reads on exons, introns and other genomic regions, average 5'-3' coverage and alignment efficiency. However, summary metrics are only part of the story. Accurate representation of transcript levels is important to any RNA-seq experiment. We present a simple interface to compare transcript levels as well as 5'-3' coverage profiles of individual transcripts across experiments. SeqResults now contains millions of individual results from 6841 libraries produced during development of NEBNext library preparation reagents.



Attendees (3)