→ Slides, doi: 10.7490/f1000research.1112913.1
This workshop will cover visualization in Galaxy for both primary high-throughput sequencing /next-generation sequencing (NGS) analyses—alignments, variants, expression levels, and annotations—as well as visualization of downstream and aggregated datasets using histograms, heat maps, and other numerical plots. First, using datasets from a combined exome and transcriptome (RNA-seq) experiment, participants will visualize data using Galaxy’s genome browser and Circos plot. Participants will learn how to create a genome visualization, add data, configure data, move between a genome browser view and Circos view, and share complex genome visualizations with more than 12 NGS datasets. Second, using an integrated datasets of genomics and other -omics information, participants will create a several numerical plots (e.g., scatter plot, histogram) to gain an overview of the data. Based on insight gained from these visualizations, participants will create a heatmap to identify patterns and potential causal factors. All visualizations will be created, saved, and shared using only Galaxy and a Web browser; no data or software downloads will be necessary.